膜杰作
Star Staining
分子别名（Synonym）
FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
表达区间及表达系统（Source）
Cynomolgus CD3 epsilon, His Tag (CDE-C5226) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1).
Predicted N-terminus: Gln 22
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.1 kDa. The protein migrates as 18-23 kDa under reducing (R) condition, and 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Cynomolgus CD3 epsilon, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) is more than 90% and the molecular weight of this protein is around 32-50 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilize Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA), and then add Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection is performed using HRP-conjugated streptavidin. Binding occurs at a linear range of 1-5 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Bispecific T-cell Engager captured on Protein A Chip can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.674 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Anti-Human CD3 mAb, mouse IgG1 (Clone # SP3541-2) on AMC Biosensor, can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.305 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Bispecific T-cell Engager (CD3 X BCMA) on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H525541), can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.113 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
