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Biotinylated Human BTLA (31-150) Protein, Fc,Avitag™ (MALS verified)

分子别名(Synonym)

BTLA,CD272

表达区间及表达系统(Source)

Biotinylated Human BTLA (31-150), Fc,Avitag (BTA-H82F3) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1).

Predicted N-terminus: Lys 31

Request for sequence

蛋白结构(Molecular Characterization)

BTLA Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

BTLA SDS-PAGE

Biotinylated Human BTLA (31-150), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

BTLA SEC-MALS

The purity of Biotinylated Human BTLA (31-150), Fc,Avitag (Cat. No. BTA-H82F3) is more than 80% and the molecular weight of this protein is around 115-145 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

BTLA ELISA

Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-150), Fc,Avitag (Cat. No. BTA-H82F3) with a linear range of 0.02-0.625 μg/mL (QC tested).

Protocol

 

背景(Background)

B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.

 

前沿进展

 
 
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BTLA靶点信息
英文全称:B- and T-lymphocyte attenuator
中文全称:B和T淋巴细胞衰减因子
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床三期
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