膜杰作
Star Staining
分子别名（Synonym）
PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
表达区间及表达系统（Source）
Rhesus macaque CD155, His Tag (CD5-R52H5) is expressed from human 293 cells (HEK293). It contains AA Asp 28 - Asn 343 (Accession # Q0MSE6-1).
Predicted N-terminus: Asp 28
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.3 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rhesus macaque CD155, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque TIGIT, Fc Tag (Cat. No. TIT-C5254) with a linear range of 0.02-0.313 μg/mL (QC tested).
Protocol
Immobilized Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DNAM-1, Fc,Avitag (Cat. No. DN1-H82F9) with a linear range of 10-156 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) on Protein A Biosensor, can bind Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) with an affinity constant of 0.47 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells.
CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains.
CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.
