膜杰作
Star Staining
分子别名（Synonym）
TPBG,5T4,M6P1,5T4AG,WAIF1,5T4 oncofetal antigen,Trophoblast glycoprotein,5T4 oncofetal trophoblast glycoprotein
表达区间及表达系统（Source）
Unconjugated Human TPBG, His,Avitag (TPG-H52E5) is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Ser 355 (Accession # Q13641-1).
Predicted N-terminus: Ser 32
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
应用说明（Application）
TPG-H52E5 is the unconjugated version of TPG-H82Eb (Biotinylated Human TPBG, His,Avitag). It can be utilized for assay development or customized biotinylation according to the experimental designs.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Unconjugated Human TPBG, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Unconjugated Human TPBG, His,Avitag (Cat. No. TPG-H52E5) at 1 μg/mL (100 μL/well) can bind Anti-TPBG Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
背景（Background）
Trophoblast glycoprotein (TPBG), also known as 5T4, is the therapeutic target of several anticancer agents currently in clinical development, largely due to its high expression in tumors and low expression in normal adult tissues.This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. TPBG is expressed by all types of trophoblasts as early as 9 weeks of development.
