膜杰作
Star Staining
分子别名（Synonym）
IL-22,IL-TIF,ZCYTO18
表达区间及表达系统（Source）
Human IL-22, His Tag (IL2-H524a) is expressed from human 293 cells (HEK293). It contains AA Ala 34 - Ile 179 (Accession # Q9GZX6-1).
Predicted N-terminus: His
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 18.6 kDa. The protein migrates as 28-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-22, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human IL-22, His Tag (Cat. No. IL2-H524a) at 5 μg/mL (100 μL/well) can bind Anti-Human IL-22 MAb with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
Immobilized Human IL-22, His Tag (Cat. No. IL2-H524a) at 5 μg/mL (100 μL/well) can bind Human IL-22 R alpha 1, Fc Tag (Cat. No. IL1-H5258) with a linear range of 2-78 ng/mL (Routinely tested).
Protocol
Immobilized Human IL-22, His Tag (Cat. No. IL2-H524a) at 5 μg/mL (100 μL/well) can bind Human IL-22BP Protein, Fc Tag (Cat. No. ILP-H5254) with a linear range of 0.2-10 ng/mL (Routinely tested).
Protocol
背景（Background）
Interleukin-22 (IL22) is also known as cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor (IL-TIF), which is belongs to the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein IL-22BP. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10.
