膜杰作
Star Staining
分子别名（Synonym）
PDGFRA,CD140A,PDGFR2,RHEPDGFRA,MGC74795
表达区间及表达系统（Source）
Human PDGF R alpha, His Tag (PDA-H52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 528 (Accession # P16234-1).
Predicted N-terminus: Gln 24
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 58.4 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human PDGF R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) at 5 μg/mL (100 μL/well) can bind HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag (Cat. No. GLO-H5283) with a linear range of 5-39 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) immobilized on CM5 Chip can bind HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag (Cat. No. GLO-H5283) with an affinity constant of 0.155 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
PDGFRA(platelet-derived growth factor receptor A), also termed PDGFRα(platelet-derived growth factor receptor α), is a receptor located on the surface of a wide range of cell types. This receptor binds to certain isoforms of platelet-derived growth factors (PDGFs) and thereby becomes active in stimulating cell signaling pathways that elicit responses such as cellular growth and differentiation. The receptor is critical for the development of certain tissues and organs during embryogenesis and for the maintenance of these tissues and organs, particularly hematologic tissues, throughout life. Mutations in the gene which codes for PDGFRA, are associated with an array of clinically significant neoplasms.
