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 >  Protein>Glypican 1 >GP1-M52H7

Mouse Glypican 1 / GPC1 Protein, His Tag

分子别名(Synonym)

Glypican 1,GPC1

表达区间及表达系统(Source)

Mouse Glypican 1, His Tag (GP1-M52H7) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 529 (Accession # Q9QZF2-1).

Predicted N-terminus: Asp 24

Request for sequence

蛋白结构(Molecular Characterization)

Glypican 1 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 57.5 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Glypican 1 SDS-PAGE

Mouse Glypican 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Glypican 1 ELISA

Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Mouse Glypican 1, His Tag (Cat. No. GP1-M52H7) with a linear range of 5-78 ng/mL (QC tested).

Protocol

 

背景(Background)

Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

 

前沿进展

 
 
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Glypican 1靶点信息
英文全称:Glypican-1
中文全称:磷脂酰肌醇聚糖1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
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