膜杰作
Star Staining
抗体来源（Source）
Biotinylated Anti-Cetuximab Antibody (AY31) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
克隆号（Clone）
AY31
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | kappa
抗体类型（Antibody Type）
Hybridoma Monoclonal
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Cetuximab.
特异性（Specificity）
Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.
纯度（Purity）
>90% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Anti-Cetuximab Antibody (AY31) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Anti-Cetuximab Antibody (12B1E9) (Cat. No.CEB-Y29c) at 2 μg/mL, add increasing concentrations of Cetuximab and then add Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No.CEB-BY31) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4.84 ng/mL (QC tested).
Protocol
Immobilized cetuximab at 0.1 μg/mL (100 μL/well) can bind Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31) with a linear range of 0.39-25 ng/mL.
Protocol
ELISA analysis shows that the binding of cetuximab to Human EGF R, His Tag (Cat. No. EGR-H5222) was inhibited by increasing concentration of Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31). The concentration of cetuximab used is 3 ng/mL.
Protocol
背景（Background）
Cetuximab is an epidermal growth factor receptor binding FAB. Cetuximab is composed of the Fv (variable; antigen-binding) regions of the 225 murine EGFr monoclonal antibody specific for the N-terminal portion of human EGFr with human IgG1 heavy and kappa light chain constant (framework) regions.
