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 >  Protein>Annexin A5 >AN5-H5123

Human Annexin A5 Protein, His Tag

分子别名(Synonym)

ANXA5,ANX5,ENX2,PP4,Annexin V,Annexin A5

表达区间及表达系统(Source)

Human Annexin A5, His Tag (AN5-H5123) is expressed from E. coli cells. It contains AA Met 1 - Asp 320 (Accession # P08758-1).

Predicted N-terminus: Met 1

Request for sequence

蛋白结构(Molecular Characterization)

Online(Met 1 - Asp 320) P08758-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.8 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>98% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Annexin A5, His Tag (Cat. No. AN5-H5123) SDS-PAGE gel

Human Annexin A5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 98%.

背景(Background)

Annexin A5 is a phospholipid binding protein, which binds with high affinity and selectivity to PS in the presence of calcium. PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Annexin A5 will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Annexin A5 has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin A5 is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).

 

文献引用(Citations)

 

前沿进展

 
 
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Annexin A5靶点信息
英文全称:Annexin A5
中文全称:膜联蛋白A5
种类:
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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