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 >  Protein>Siglec-9 >SI9-H5256

Human Siglec-9 Protein, Fc Tag

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分子别名(Synonym)

Siglec-9,SIGLEC9,CDw329,CD329

表达区间及表达系统(Source)

Human Siglec-9, Fc Tag (SI9-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 348 (Accession # Q9Y336-1).

Predicted N-terminus: Gln 18

Request for sequence

蛋白结构(Molecular Characterization)

Siglec-9 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 62.5 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Siglec-9 SDS-PAGE

Human Siglec-9, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Siglec-9 ELISA

Immobilized Human Siglec-9, Fc Tag (Cat. No. SI9-H5256) at 10 μg/mL (100 μL/well) on Diamond Protein A Protein, His Tag precoated (0.5 μg/well) plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.156-2.5 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Siglec-9 BLI

Loaded Human Siglec-9, Fc Tag (Cat. No. SI9-H5256) on ProteinA Biosensor, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with an affinity constant of 0.85 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

siglec-9 (HGMW-approved symbol SIGLEC9) a member of the sialic acid-binding Ig-like lectin (Siglec) family, which belongs to the immunoglobulin superfamily (IgSF). SIGLEC9 shows a high degree of homology to many members of the siglec family, including siglec-7 (80%), siglec-8 (72%), siglec-5 (65%), and CD33 (64%). This high degree of homology is also conserved in the extracellular Ig-like domains. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains。Siglec-9 with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail.

 

前沿进展

 
 
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Siglec-9靶点信息
英文全称:Sialic acid-binding Ig-like lectin 9
中文全称:唾液酸结合免疫球蛋白样凝集素-9
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:
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