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 >  Protein>IL-17A >ILA-H82Q1

Biotinylated Human IL-17A / CTLA-8 Protein, His,Avitag™

分子别名(Synonym)

IL-17A,Interleukin-17A,CTLA-8,IL-17

表达区间及表达系统(Source)

Biotinylated Human IL17A, His,Avitag (ILA-H82Q1) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (Accession # Q16552-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

IL-17A Structure

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 17.8 kDa. The protein migrates as 18-21 kDa and 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-17A SDS-PAGE

Biotinylated Human IL17A, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

IL-17A ELISA

Immobilized Human IL-17 RA, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL17A, His,Avitag (Cat. No. ILA-H82Q1) with a linear range of 0.3-10 ng/mL (QC tested).

Protocol

IL-17A ELISA

Immobilized Monoclonal Anti-Human IL-17A Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL17A, His,Avitag (Cat. No. ILA-H82Q1) with a linear range of 0.3-2 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

IL-17A BLI

Loaded Biotinylated Human IL17A, His,Avitag (Cat. No. ILA-H82Q1) on SA Biosensor, can bind Human IL-17 RA, His Tag (Cat. No. ILA-H5222) with an affinity constant of 18.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

 

前沿进展

 
 
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IL-17A靶点信息
英文全称:Interleukin 17A
中文全称:白细胞介素-17A
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:29详情
最高研发阶段:批准上市
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