膜杰作
Star Staining
分子别名（Synonym）
Serum albumin,ALB,Alb
表达区间及表达系统（Source）
Biotinylated Human Serum Albumin, His,Avitag (HSA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Leu 609 (Accession # P02768-1).
Predicted N-terminus: Asp 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 70.2 kDa. The protein migrates as 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Biotinylated Human Serum Albumin, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Serum Albumin, His,Avitag (Cat. No. HSA-H82E3) is more than 90% and the molecular weight of this protein is around 64-80 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-BLI
Loaded Biotinylated Human Serum Albumin, His,Avitag (Cat. No. HSA-H82E3) on SA Biosensor, can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) with an affinity constant of 0.641 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
+添加评论
- 203XXXXXXX
- My biotinylated Human Serum Albumin came in a timely manner, which was crucial for my experiment to run so I could meet deadlines. I was happy to find out that AcroBiosystems offered a biotinylated product that was greater than 95% purity. I ordered the 25 ug HSA which worked well for my purposes for SPR by the Biacore T200. My shipment arrived perfectly and it was packaged safely. I would order from this company again for their quick processing time and quality of products.
- 2022-3-21
- 183XXXXXXX9
- 该蛋白是生物素化的修饰蛋白，我们利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性，将生物素化的HSA蛋白与链霉亲和素产品联合应用于ELISA实验，与非生物素体系相比，检测速度更快、结合能力更强、非特异背景更低。已经使用该蛋白进行了一年多的ELISA实验，获得很好的实验结果。此外，ACRO的发货速度和服务态度很赞，会继续使用该蛋白进行产品的稳定性能研究。
- 2023-10-7
- 183XXXXXXX9
- 该蛋白属于生物素化蛋白，具有高特异性和高灵敏度的结合特性，我们主要将该生物素化蛋白应用于Binding ELISA实验，与非生物素体系相比，检测速度更快、结合能力更强、非特异背景更低。
- 2023-2-14
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背景（Background）
serum albumin (SA) is also known as ALB, which is the main protein of plasma and has a good binding capacity for water,Ca2+,Na+,K+,fatty acids,hormones, bilirubin and drugs.The main function of SA is the regulation of the colloidal osmotic pressure of blood. As Major zinc transporter in plasma, SA typically binds about 80% of all plasma zinc. A variant structure of albumin could lead to increased binding of zinc resulting in an asymptomatic augmentation of zinc concentration in the blood. Defects in serum albumin can cause familial dysalbuminemic hyperthyroxinemia which is a form of euthyroid hyperthyroxinemia that is due to increased affinity of serum albumin for T4. It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
