膜杰作
Star Staining
分子别名（Synonym）
FGL1,Hepassocin,HP-041,HFREP-1,LFIRE-1,HFREP1
表达区间及表达系统（Source）
Biotinylated Human FGL1, Avitag,Fc Tag (FG1-H82F4) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Ile 312 (Accession # Q08830-1).
Predicted N-terminus: Gly
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 62.1 kDa. The protein migrates as 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human FGL1, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human LAG-3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. LA3-H525c) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGL1, Avitag,Fc Tag (Cat. No. FG1-H82F4) with a linear range of 0.156-5 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Human FGL1, Avitag,Fc Tag (Cat. No. FG1-H82F4) at 5 μg/mL (100 μL/well)on streptavidin (Cat. No. STN-N5116) (2 μg/well) plate. can bind Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) with a linear range of 0.156-2.5 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human FGL1, Avitag,Fc Tag (Cat. No. FG1-H82F4) on SA Biosensor, can bind Human LAG-3, Fc Tag (Cat. No. LA3-H5255) with an affinity constant of 4.12 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-FACS
FACS analysis shows that Biotinylated Human FGL1, Avitag,Fc Tag (Cat. No. FG1-H82F4) can bind to 293T cells overexpressing human LAG3. The concentration of Human FGL1 is 10 μg/mL (Routinely tested).
Protocol
背景（Background）
Fibrinogen-like protein 1(FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1. The protective effect of fibrinogen-like protein 1 (FGL1) in liver injury has previously been reported. However, studies have shown that FGL1 may be a predictor of GC patients and a target for GC therapy. Immunocytochemical studies revealed that fgl1 selectively binds to defective spermatozoa in the cauda epididymidis. Northern blot analysis and in situ hybridization demonstrated the high expression of fgl1 in the principal cells of the proximal cauda epididymidis. Immunofluorescence analysis using mouse fibrotic lung tissues suggested that fibrotic regions showed increased expressions of Gtse1 and Fgl1, Gtse1 and Fgl1 are suggested to be novel targets for radiation-induced lung fibrosis.
