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Human CD7 Protein, Llama IgG2b Fc Tag, low endotoxin

分子别名(Synonym)

CD7,GP40,TP41,LEU-9,Tp40

表达区间及表达系统(Source)

Human CD7 Protein, Llama IgG2b Fc Tag (CD7-H5258) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1).

Predicted N-terminus: Glu

Request for sequence

蛋白结构(Molecular Characterization)

CD7 Structure

This protein carries a llama IgG2b Fc tag at the N-terminus.

The protein has a calculated MW of 44.3 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系(QMS)

  1. 质量管理体系(ISO, GMP)
  2. 质量优势
  3. 质控流程
 

电泳(SDS-PAGE)

CD7 SDS-PAGE

Human CD7 Protein, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

CD7 ELISA

Immobilized Human CD7 Protein, Llama IgG2b Fc Tag (Cat. No. CD7-H5258) at 5 μg/mL (100 μL/well) can bind Human SECTM1, His Tag (Cat. No. SE1-H5227) with a linear range of 10-39 ng/mL (QC tested).

Protocol

 
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背景(Background)

T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

 

前沿进展

Genetic Characterization and Zoonotic Analyses of Enterocytozoon bieneusi from Cats and Dogs in Shanghai in China
Zhang, Zhang, Mi et al
Vector Borne Zoonotic Dis (2025)
Abstract: Background: Enterocytozoon bieneusi is reported to be a common microsporidian of humans and animals in various countries. However, limited information on E. bieneusi has been recorded in cats (Felis catus) and dogs (Canis familiaris) in China. Here, we undertook molecular epidemiological investigation of E. bieneusi in cats and dogs in Shanghai, China. Methods: A total of 359 genomic DNAs were extracted from individual fecal samples from cats (n = 59) and dogs (n = 300), and then were tested using a nested PCR-based sequencing approach employing internal transcribed spacer (ITS) of nuclear ribosomal DNA as the genetic marker. Results: Enterocytozoon bieneusi was detected in 34 of 359 (9.5%) (95% confidence interval [6.7 - 13.0%]) fecal samples from cats (32.2%; 19/59) and dogs (5.0%; 15/300), including 24 stray cats and dogs (22.6%; 24/106), as well as 10 household/raised cats and dogs (4.0%; 10/253). Correlation analyses revealed that E. bieneusi positive rates were significantly associated with stray cats and dogs (p < 0.05). The analysis of ITS sequence data revealed the presence of five known genotypes, CD7, CHN-HD2, D, PtEb IX, and Type IV, and two novel genotypes, D-like1 and PtEb IX-like1. Zoonotic genotype D was the predominant type with percentage of 61.8% (21/34). Phylogenetic analysis of ITS sequence data sets showed that genotypes D, D-like1, and Type IV were clustered within Group 1, showing zoonotic potential. The others were assigned into Group 10 with host specificity. Conclusions: These findings suggested that cats and dogs in Shanghai harbor zoonotic genotype D of E. bieneusi and may have a significant risk for zoonotic transmission. Further insight into the epidemiology of E. bieneusi in other animals, water, and the environment from other areas in China will be important to have an informed position on the public health significance of microsporidiosis caused by this microbe.
Analysis of Antigen Expression in T-Cell Acute Lymphoblastic Leukemia by Multicolor Flow Cytometry: Implications for the Detection of Measurable Residual Disease
Semchenkova, Mikhailova, Demina et al
Int J Mol Sci (2025) 26 (5)
Abstract: Multicolor flow cytometry (MFC) is a key method for assessing measurable residual disease (MRD) in acute lymphoblastic leukemia (ALL). However, very few approaches were developed for MRD in T-cell ALL (T-ALL). To identify MRD markers suitable for T-ALL, we analyzed the expression of CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD34, CD45, CD48, CD56, CD99, and HLA-DR in T-ALL patients at diagnosis. The median fluorescence intensities (MFIs) of surface CD3, CD4, CD5, CD7, CD8, CD45, CD48, CD99, and CD16+CD56 were also evaluated at Day 15 and the end-of-induction (EOI). The MFC data from 198 pediatric T-ALL patients were analyzed retrospectively. At diagnosis, the most common antigens were identified, and the MFI of T-lineage antigens in blasts was compared to that in T lymphocytes. At follow-up, the MFIs of the proposed MRD markers were compared to those observed at diagnosis. The most common T-ALL antigens were CD7 (100.0%), intracellular CD3 (100.0%), CD45 (98.5%), and CD5 (90.9%). The MFIs of T-lineage antigens in blasts differed significantly from those in T lymphocytes. By the EOI, a substantial modulation of sCD3, CD4, CD5, CD7, CD8, and CD45 was observed. CD48 and CD99 were the most stable markers. The proposed MRD markers (sCD3, CD4, CD5, CD7, CD8, CD45, CD48, CD99, CD16+CD56) enabled MFC-MRD monitoring in virtually all T-ALL patients.
CD7 CAR-T: a bridge to transplant in AML
Mamonkin
Blood (2025) 145 (10), 995-996
Showing 1-4 of 2822 papers.
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CD7靶点信息
英文全称:T-cell antigen CD7
中文全称:T细胞抗原CD7
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:23详情
最高研发阶段:临床二期
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