膜杰作
Star Staining
分子别名（Synonym）
Mucin 1,MUC1,CD227,EMA,H23AG,KL-6,MAM6,MUC-1,SEC,MUC-1,X,MUC1,ZD,PEM,PEMT,PUM,CA15-3,Episialin
表达区间及表达系统（Source）
Human Mucin-1 (890-1158), His Tag (MU1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ser 890 - Gly 1158 (Accession # P15941-1).
Predicted N-terminus: Ser 890 (partial α chain) & Ser 1098 (partial β chain)
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 21.3 kDa (partial α chain) and 8.4 kDa (partial β chain). The protein migrates as 40-80 kDa (partial glycosylated α chain) and 10 kDa (partial β chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Mucin-1 (890-1158), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Mucin-1 (890-1158), His Tag (Cat. No. MU1-H52H9) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Mucin-1 / MUC-1 (890-1158) Protein, His Tag (Cat. No. MU1-H52H9) at 1 μg/mL (100 μL/well) can bind Anti-Mucin-1 Antibody, Mouse IgG1 with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
背景（Background）
Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. Interacts via its C-terminus with PDZK1 and this interaction appears important for proper localization. Probably plays a role in maintaining homeostasis on mucosal surfaces.
