膜杰作
Star Staining
分子别名（Synonym）
Mucin 1,MUC1,CD227,EMA,H23AG,KL-6,MAM6,MUC-1,SEC,MUC-1,X,MUC1,ZD,PEM,PEMT,PUM,CA15-3,Episialin
表达区间及表达系统（Source）
Human Mucin-1 (1036-1155), His Tag (MU1-H52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 1036 - Gly 1155 (Accession # P15941-1).
Predicted N-terminus: Leu 1036 (partial α chain) & Ser 1098 (partial β chain)
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 7.2 kDa (partial α chain) and 8.2 kDa (partial β chain). The protein migrates as 13-15 kDa (partial α chain) and 10 kDa (partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Human Mucin-1 (1036-1155), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human Mucin-1 (1036-1155), His Tag (Cat. No. MU1-H52H5) at 1 μg/mL (100 μL/well) can bind Anti-Mucin-1 Antibody, Mouse IgG1 with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of anti-Mucin-1 CAR-293 were stained with 100 μL of 0.1 μg/mL of Human Mucin-1, His Tag (Cat. No. MU1-H52H5) and negative control protein respectively, washed and then followed by PE anti-His Tag antibody and analyzed with FACS (Routinely tested).
Protocol
+添加评论
- 188XXXXXXX0
- Human MUC-1 Protein是一种重要的糖蛋白，在上皮细胞保护和细胞信号传导中发挥关键作用。采购该蛋白主要用于定量检测细胞培养液中的MUC-1水平，蛋白活性高，用低检测量即可达到检测要求，节约成本，性价比高，很值得购买。
- 2024-4-28
- 150XXXXXXX2
- Human Mucin-1这款蛋白的活性很稳定，最初其动物免疫学数据也很不错，后期表达验证ELISA及SPR数据可信度均很高。ACRO是我们的长期合作伙伴，其售后服务也是一流的，值得信赖！
- 2024-4-30
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背景（Background）
Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. Interacts via its C-terminus with PDZK1 and this interaction appears important for proper localization. Probably plays a role in maintaining homeostasis on mucosal surfaces.
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