登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>IL-13 >IL3-H82E5

Biotinylated Human IL-13 Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,Interleukin-13

表达区间及表达系统(Source)

Biotinylated Human IL-13, His,Avitag (IL3-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).

Predicted N-terminus: Gly 21

Request for sequence

蛋白结构(Molecular Characterization)

IL-13 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 15.9 kDa. The protein migrates as 17 kDa and 28-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-13 SDS-PAGE

Biotinylated Human IL-13, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

IL-13 SEC-MALS

The purity of Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) is more than 85% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-13 ELISA

Immobilized Human IL-13 R alpha 2, Fc Tag (Cat. No. IL2-H5256) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) with a linear range of 0.2-5 ng/mL (QC tested).

Protocol

IL-13 ELISA

Immobilized Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human IL-13 Antibody, Human IgG4 with a linear range of 0.3-20 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

IL-13 BLI

Loaded Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) on SA Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) with an affinity constant of 6.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
IL-13靶点信息
英文全称:Interleukin-13
中文全称:白细胞介素-13
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:9详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定