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 >  Protein>Apolipoprotein E >APE-H5246

Human Apolipoprotein E / APOE3 Protein, His Tag

分子别名(Synonym)

Apolipoprotein E,APOE,Apo-E

表达区间及表达系统(Source)

Human APOE3, His Tag (APE-H5246) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P02649-1).

Predicted N-terminus: Lys 19

Request for sequence

蛋白结构(Molecular Characterization)

Apolipoprotein E Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.1 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

无菌(Sterility)

Negative

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Apolipoprotein E SDS-PAGE

Human APOE3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-BLI

Apolipoprotein E BLI

Loaded Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) on SA Biosensor, can bind Human APOE3, His Tag (Cat. No. APE-H5246) with an affinity constant of 37.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Apolipoprotein E BLI

Loaded Human TREM2, Fc Tag (Cat. No. TR2-H5254) on Protein A Biosensor, can bind Human APOE3, His Tag (Cat. No. APE-H5246) with an affinity constant of 66.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Apolipoprotein E BLI

Loaded Human TREM2, Mouse IgG2a Fc Tag (Cat. No. TR2-H5256) on Protein A Biosensor, can bind Human APOE3, His Tag (Cat. No. APE-H5246) with an affinity constant of 9.53 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

Apolipoprotein E FACS

3e5 of HeLa cells were co-incubated with 50 nM Cy3-labeled LNPs-siRNA in the presence or absence of 1 μg/mL Human APOE3, His Tag (Cat. No. APE-H5246), washed and analyzed with FACS. PE signal was used to evaluate the expression of Cy3+ HeLa cells (Routinely tested).

Protocol

 

背景(Background)

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles.

 

前沿进展

 
 
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Apolipoprotein E靶点信息
英文全称:Apolipoprotein E
中文全称:载脂蛋白E
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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