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 >  Protein>NKG2A & CD94 >NC4-H5253

Human NKG2A&CD94 Protein, Mouse IgG2a Fc Tag (MALS verified)

分子别名(Synonym)

NKG2A & CD94

表达区间及表达系统(Source)

Human NKG2A&CD94, Mouse IgG2a Fc Tag (NC4-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 113 - Leu 233 (NKG2A) & Asp 57 - Ile 179 (CD94) (Accession # P26715-1 (NKG2A) & Q13241-1 (CD94)).

Predicted N-terminus: Ala 113

蛋白结构(Molecular Characterization)

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 55.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

NKG2A & CD94 SDS-PAGE

Human NKG2A&CD94, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

NKG2A & CD94 SEC-MALS

The purity of Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) is more than 90% and the molecular weight of this protein is around 125-145 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

NKG2A & CD94 ELISA

Immobilized Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-NKG2A (Z270), Human IgG1 with a linear range of 0.2-8 ng/mL (QC tested).

Protocol

NKG2A & CD94 ELISA

Immobilized Human NKG2A&CD94, Mouse IgG2a Fc Tag (Cat. No. NC4-H5253) at 5 μg/mL (100 μL/well) can bind various dilution ratio of HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE (Routinely tested).

Protocol

 

背景(Background)

CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. NKG2A/CD159a is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors that inhibits innate immune system activation. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA)-E on target cells.

 

前沿进展

 
 
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NKG2A & CD94靶点信息
英文全称:NK cell lectin-like receptor subfamily C member 1
中文全称:自然杀伤细胞凝集素样受体亚家族C成员1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床三期
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