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 >  Protein>Activin RIB >ACB-H52H4

Human Activin RIB / ACVR1B Protein, His Tag

分子别名(Synonym)

ACVR1b,ALK4,ACVRLK4,ACTR-IB,SKR2

表达区间及表达系统(Source)

Human Activin RIB, His Tag (ACB-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Glu 126 (Accession # P36896-1).

Predicted N-terminus: Ser 24

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ser 24 - Glu 126) P36896-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 13.3 kDa. The protein migrates as 16-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Activin RIB, His Tag (Cat. No. ACB-H52H4) SDS-PAGE gel

Human Activin RIB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human TDGF1, Fc Tag on Protein A Biosensor, can bind Human Activin RIB, His Tag (Cat. No. ACB-H52H4) with an affinity constant of 5.6 μM as determined in BLI assay (ForteBio Octet Red96e) (QC Tested).

Protocol

 

背景(Background)

Activin receptor type-1B (ACVR1B) is also known as Activin receptor-like kinase 4 (ALK-4), Serine/threonine-protein kinase receptor R2 (SKR2), which belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. ACVR1B contains one GS domain and one protein kinase domain. The catalytic activity of ACVR1B is “ATP + [receptor-protein] = ADP + [receptor-protein] phosphate”. ACVR1B acts as a transducer of activin or activin like ligands (e.g., inhibin) signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. These go on to recruit the R-SMADs SMAD2 or SMAD3. ACVR1B also transduces signals of nodal, GDF-1, and Vg1.

 

 

前沿进展

 
 
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Activin RIB靶点信息
英文全称:Transforming growth factor beta receptor 1
中文全称:转化生长因子β受体1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:8详情
最高研发阶段:临床二期
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