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Cynomolgus ACE2 / ACEH Protein, His Tag

分子别名(Synonym)

ACE-2,ACEH,ACE2

表达区间及表达系统(Source)

Cynomolgus ACE2, His Tag (AC2-C52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Thr 741 (Accession # A0A2K5X283-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

ACE2 Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 85.7 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

ACE2 SDS-PAGE

Cynomolgus ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

ACE2 ELISA

Immobilized Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) with a linear range of 2-78 ng/mL (QC tested).

Protocol

ACE2 ELISA

Immobilized Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E8) with a linear range of 0.005-0.313 μg/mL (Routinely tested).

Protocol

ACE2 ELISA

Immobilized Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with a linear range of 0.4-13 ng/mL (Routinely tested).

Protocol

ACE2 ELISA

Immobilized Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) with a linear range of 0.1-0.8 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

ACE2 BLI

Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) with an affinity constant of 4.48 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

ACE2 BLI

Loaded SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) on Protein A Biosensor, can bind Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) with an affinity constant of 3.06 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

文献引用(Citations)

 

前沿进展

 
 
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ACE2靶点信息
英文全称:Angiotensin-converting enzyme 2
中文全称:血管紧张素转换酶 2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:7详情
最高研发阶段:临床二期
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