膜杰作
Star Staining
分子别名（Synonym）
SIGLEC10,MGC126774,PRO940,Siglec10,SLG2
表达区间及表达系统（Source）
PE-Labeled Human Siglec-10, His Tag (SI0-HP2E5) is produced via site-specific conjugation of PE to Human Siglec-10, His Tag under optimal conditions with a proprietary technology with a proprietary technology. Human Siglec-10, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
Predicted N-terminus: Met 17
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 61.8 kDa.
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
应用说明（Application）
Flow Cytometry (Neutralizing assay), Please note that this product is NOT compatible to streptavidin detection system.
Designed and validated to use together with CD24 overexpressing HEK293 cells.
Not suitable to use together with overexpressing CHO cells for high non-specific background from proteins of high sialylation.
Not recommended and validated to use together with tumor cells.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS with Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
活性（Bioactivity）-FACS
2e5 of 293 cells overexpressing human CD24 were stained with 100μL of 1:10 dilution of PE-Labeled Human Siglec-10 Protein, His Tag(recommended for neutralizing assay)(Cat. No. SI0-HP2E5)and negative control protein respectively , washed and analyzed with FACS (QC tested).
Protocol
背景（Background）
The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
