Human BMP-2 Protein, premium grade

Abstract: Currently, bone defects remain a major challenge in clinical treatment. Recruiting target cells at the defect site and inducing them to differentiate into bone tissue are effective treatment methods. In previous studies, we used the CD271 antibody to construct bone marrow mesenchymal stem cell (BMSC) recruitment microspheres for the treatment of bone defects. However, the osteoconductivity of the microspheres themselves was poor, and the system lacked osteoinductivity, which affected the repair efficiency. In this study, we prepared submillimeter-sized porous chitosan (CS) microspheres through process optimization, and the BMSCs were able to directly adhere and proliferate on their surfaces. After the bioconjugation of the CD271 antibody, bone morphogenetic protein-2 (BMP-2) was further loaded onto the pore structure of microspheres to obtain the injectable microspheres with BMSC recruitment and osteogenic differentiation induction functions. Microspheres could efficiently recruit BMSCs through the combined action of the CD271 antibody and BMP-2 and further induce the recruited BMSCs, differentiating into osteoblasts through BMP-2, which ultimately exhibited promising bone regeneration ability in rats. We expect that the novel functional microspheres have great potential in biomedical applications for in situ treatment of bone defects.

Abstract: This study explored the interaction effects of dietary Vitamin A (VA) and Vitamin D3 (VD3) on growth performance, jejunal function, and tibia development in goslings, aiming to identify any synergistic outcomes that may reshape nutritional strategies for geese production. A total of 540 one-day-old male Jiangnan White goslings with similar body weight (82 ± 5 g) were randomly assigned into 9 treatments with five replicate pens per treatment and 12 birds per pen. The bird trial employed a 3 × 3, two-factorial treatment with three levels of VA (5000, 7000, and 9000 IU/kg) and three levels of VD3 (1000, 1500, and 2000 IU/kg) from one to 28 days of age. Main effects analysis indicated that birds fed 7000 IU/kg VA exhibited the highest ADG, BW, jejunal maltase activity and IL-10 content (P < 0.05), while 9000 IU/kg VA had the highest SOD activity and content of IL-6 and TNF-α in jejunal mucosa (P < 0.05). Both 7000 IU/kg or 9000 IU/kg VA increased the jejunal IL-1β content, relative expression of tight junction protein 1 (TJP1) mRNA, tibia defatted weight and ash weight (P < 0.05). Birds fed 2000 IU/kg VD3 exhibited the highest ADFI, while both 1500 or 2000 IU/kg VD3 increased jejunal maltase activity, and tibia ash content (P < 0.05). An interaction between VA and VD3 on ADFI, F/G, jejunal maltase activity, mucosal immune factors (IL-1β, IL-6, IL-10, TNF-α), tibia ash content, and bone morphogenetic protein-2 (BMP-2) expression. A simple effects analysis revealed that at a 5000 IU/kg VA, adding 1000 IU/kg VD3 decreased IL-1β, IL-6, TNF-α (P < 0.05). At a 7000 IU/kg VA, adding 1500 or 2000 IU/kg VD3 decreased TNF-α, and increased jejunal maltase activity(P < 0.05). At a 9000 IU/kg VA, adding 1000 IU/kg VD3 decreased ADFI, F/G, jejunal maltase activity, tibia ash, and BMP-2, while IL-1β, IL-6, and TNF-α increased (P < 0.05). At a 9000 IU/kg VA, adding 2000 IU/kg VD3 increased IL-10 (P < 0.05). At a 1000 IU/kg VD3, adding 5000 IU/kg VA increased F/G, jejunal maltase activity and IL-10, while decreased IL-1β, IL-6, TNF-α (P < 0.05), and adding 9000 IU/kg VA decreased tibia ash and BMP-2 (P < 0.05). At 1500 or 2000 IU/kg VD3, adding 7000 IU/kg VA increased jejunal maltase activity, IL-10 (P < 0.05). At a 2000 IU/kg VD3, adding 9000 IU/kg VA increased IL-6, and TNF-α (P < 0.05). In summary, a dietary level of 7000 IU/kg of VA and 2000 IU/kg of VD3 can be a balanced combination to optimize feed intake and conversion, jejunal function, and tibia mineralization, consequently enhancing growth performance in goslings.Copyright © 2025. Published by Elsevier Inc.

Abstract: This case report describes the use of a recombinant human bone morphogenetic protein-2 (rhBMP-2)-coated dental implant for immediate placement in a 65-year-old male with a nonrestorable maxillary right first premolar. Due to the compromised condition of the tooth, extraction was necessary, and immediate implant placement was considered appropriate based on the patient's dental and overall health. The rhBMP-2-coated implant was selected to enhance bone regeneration and promote osseointegration in the severely resorbed alveolar ridge. Over a six-month follow-up period, the patient exhibited successful implant integration, minimal postoperative complications, and favorable functional and aesthetic outcomes.Copyright © 2025, Kaushik et al.

Abstract: In recent years, hyaluronic acid (HA) and the natural tripeptide glycyl-l-histidyl-l-lysine (GHK), especially its copper(II) complex (GHK-Cu), individually have been shown to exert helpful properties for bone protection and regeneration. However, they are not strong enough to handle oxidative stress, hydrolytic attack, or environmental conditions. Being aware that conjugation chemistry has recently emerged as an appealing approach for generating new molecular entities capable of preserving the molecular integrity of their moieties or delaying their degradation, herein we present the synthesis of conjugates of HA with GHK (GHK-HA), at different loadings of the tripeptide. GHK-HA binds copper(II) ions and potentiates the chemical and biological properties of the two components in in vitro assays. The results highlight copper's role in promoting the expression and release of certain trophic, angiogenic, and osteogenic factors, including brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), as well as bone morphogenetic protein-2 (BMP-2). The protective and regenerative activities of the metal ion are related to the translocation of its intracellular chaperones Copper Chaperone for Superoxide Dismutase (CCS) and Antioxidant-1 (Atox1) to the nucleus where they act as transcription factors.