构成成分(Composition)
This 200x detergent solution contains 10%(w/v) DDM and 2% (w/v) CHS that is necessary for solubilization of membrane proteins. DDM, or n-Dodecyl β-D-Maltoside [CAS: 69227-93-6], is a maltoside based non-ionic detergent with a hydrophilic maltose head and a hydrophobic long chain alkyl tail. CHS, or Cholesteryl Hemisuccinate Tris Salt [CAS: 102601-49-0], is an ionizable anionic detergent that stabilizes large unilamellar vesicles. DDM/CHS micelles provide a more membrane-like environment for solubilization of membrane proteins, and help to keep structural integrity and activity. This 200x detergent solution is sterile-filtered and contains no other preservatives.
应用说明(Application)
The 200x DDM/CHS solution is INDISPENSABLE to maintain solubilization and activity of membrane proteins (e.g. Full length CD20 protein, CD0-H52H3, CD0-H82E5, and CD133 protein CD3-H55H7), and should be used together with membrane proteins to dilute membrane proteins or antibodies, to prepare running buffer in SPR or BLI assay, etc. The 200x DDM/CHS solution should be diluted with assay buffer solutions to prepare 1x working solution just before experiment.
存储(Storage)
Store this solution at 2-8℃, and protect from light. If precipitation occurs, filter to clarify before use. This Solution is stable after storage at 2-8℃ no more than 3 months upon receipt.
质量管理控制体系(QMS)


- 187XXXXXXX1
- 该产品应用于亲和力和Elisa筛选试验,使用推荐200倍稀释后可以有效稳定分析物构象,帮助产生优质的结合曲线。对于CD20以及一些跨膜蛋白有非常显著的效果。
- 2024-7-20