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Human CD33-coupled Magnetic Beads

热销产品推荐:The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈1827 pmol (53.7 μg) CD33/mg Beads≈1827 pmol (53.7 μg) CD33/mg Beads
Binding Capacity>267 pmol (40 μg) antibody/mg beads>267 pmol (40 μg) antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)

背景(Background)

The biotinylated CD33 protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD33 antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This CD33 coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.

表达区间及表达系统(Source)

Human CD33 protein is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259(Accession # P20138-1 (R69G)).

应用说明(Application)

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

*The Isotype control (Cat. No. SMB-B01) is sold separately.

重构方法(Reconstitution)

See Certificate of Analysis (CoA) for detailed instruction.

存储(Storage)

Upon receipt, please store the beads at -20°C. The shelf life is 1 year at -20 °C.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

原理(Assay Principles)

Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

制剂(Formulation)

Please contact us for detailed information.

Contact us for customized product form or formulation.

 

典型数据-Typical Data

Siglec-3 TYPICAL DATA

Immobilized 53.7 μg CD33 protein to 1 mg Beads, can bind the Anti-CD33 Antibody with an EC50 of 0.7794 μg/mL.

Protocol

 

前沿进展

 
 
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Siglec-3靶点信息
英文全称:Myeloid cell surface antigen CD33
中文全称:髓系细胞表面抗原CD33
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:34详情
最高研发阶段:批准上市
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