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 >  Protein>Siglec-15 >SG5-H5254

Human Siglec-15 / CD33L3 Protein, Mouse IgG2a Fc Tag (MALS verified)

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分子别名(Synonym)

CD33 antigen-like 3,SIGLEC-15,CD33L3,sialic acid-binding Ig-like lectin 15,Siglec15,Siglec-15

表达区间及表达系统(Source)

Human Siglec-15, Mouse IgG2a Fc Tag (SG5-H5254) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).

Predicted N-terminus: Phe 20

Request for sequence

蛋白结构(Molecular Characterization)

Online(Phe 20 - Thr 263) Q6ZMC9-1

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 53.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Siglec-15, Mouse IgG2a Fc Tag (Cat. No. SG5-H5254) SDS-PAGE gel

Human Siglec-15, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human Siglec-15, Mouse IgG2a Fc Tag (Cat. No. SG5-H5254) MALS images

The purity of Human Siglec-15, Mouse IgG2a Fc Tag (Cat. No. SG5-H5254) is more than 90% and the molecular weight of this protein is around 115-130 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Siglec-15, Mouse IgG2a Fc Tag (Cat. No. SG5-H5254) at 5 μg/mL (100 μL/well) on Diamond Protein A Protein, His Tag precoated (0.5 μg/well) plate can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.02-0.313 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human Siglec-15, Mouse IgG2a Fc Tag (Cat. No. SG5-H5254) at 1 μg/mL (100 μL/well) can bind Mouse Human chimeric mAb (5G12, Human IgG1) with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

背景(Background)

Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

 

 

前沿进展

 
 
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Siglec-15靶点信息
英文全称:Sialic acid-binding Ig-like lectin 15
中文全称:唾液酸结合免疫球蛋白样凝集素-15
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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