膜杰作
Star Staining
抗体来源（Source）
Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS41) (NUN-S41) is isolated from a SARS-CoV-2 infected patient and is recombinantly produced from human 293 cells (HEK293). As verified by binding test with N-NTD (Cat. No. NUN-C5143) and N-CTD (Cat. No. NUN-C5145) protein, this antibody can only bind to N-CTD (AA Ser 255 - Pro 364). ELISA test validated that this antibody can bind multiple N protein variants (Cat. No. NUN-C52H8, NUN-C52Hc, NUN-C52Hd) with similar affinity as compared to the wild type N protein (Cat. No. NUN-C5227).
克隆号（Clone）
AS41
亚型（Isotype）
Human IgG1 | Kappa
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
特异性（Specificity）
This product can recognize SARS-CoV-2 and SARS-CoV Nucleocapsid protein. No cross-reactivity is detected with nucleocapsid protein of other coronaviruses, including MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-10 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS41) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS41) (Cat. No. NUN-S41) with a linear range of 0.08-1 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS41) (Cat. No. NUN-S41) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid protein, His Tag (B.1.1.529/Omicron) (Cat. No. NUN-C52Ht) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
- 176XXXXXXX0
- 从技术服务知道了这家公司，我们在多项目上委托了BLI服务，服务体验很好，数据及报告做的很漂亮。后续就从百普赛斯直接购买了蛋白，实验数据稳定，蛋白纯度及实验响应比较出色，供货稳定。成为了我们的关键试剂。
- 2022-9-5
- 156XXXXXXX8
- 我们采购该蛋白用于检测自主开发的抗TROP-2抗体的亲和力，通过OCTET检测其亲和动力学，我们用已经获批的IMMU132进行了测试，其亲和动力学常数为nM级别(1.05nM)。
- 2022-4-6
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背景（Background）
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
