膜杰作
Star Staining
& Cat.No. | Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Physical appearance | Powder mixture | Powder mixture |
| Amount of Coupled Protein | ≈172 pmol (15 μg) ACE2/mg Beads | ≈172 pmol (15 μg) ACE2/mg Beads |
| Binding Capacity | >20 μg Spike S1/mg beads; >20 μg Spike RBD/mg beads | >20 μg Spike S1/mg beads; >20 μg Spike RBD/mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
背景（Background）
The biotinylated ACE2 protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the SARS-CoV-2 S1 protein/SARS-CoV-2 S protein RBD from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This Human ACE2 coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
表达区间及表达系统（Source）
Human ACE2 protein is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740(Accession # Q9BYF1-1).
应用说明（Application）
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Application Method: a) Reconstitute the Beads following the COA. Wash and re-suspend the beads to a certain concentration by adding your dilution buffer. b) Add the prepared beads to your samples. c) Beads can be separated from your samples afterwards using a magnetic plate.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data
Immobilized 15 μg ACE2 protein to 1 mg Beads, can bind the SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with an EC50 of 1.402 μg/mL.
Protocol
Immobilized 15 μg ACE2 protein to 1 mg Beads, can bind the SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) with an EC50 of 0.5095 μg/mL.
Protocol
