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Human Anti-SARS-CoV-2 Spike RBD Antibody-coupled Magnetic Beads

热销产品推荐:The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈972 pmol (73.1 μg) Anti-RBD antibody/mg beads≈972 pmol (73.1 μg) Anti-RBD antibody/mg beads
Binding Capacity>192 pmol (10 μg) Spike RBD/mg beads>192 pmol (10 μg) Spike RBD/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)

背景(Background)

The Human Anti-SARS-CoV-2 Spike RBD Antibody-coupled Magnetic Beads is produced by coupling biotinylated anti-RBD antibody to streptavidin-conjugated magnetic beads. The pre-coupled beads are ready to use for capturing spike protein from your sample with high specificity. The uniform size and large surface-to-volume ratio of the beads ensure highly efficent immunocapture in a simple, fast and convenient workflow with high reproducibilty of data.

应用说明(Application)

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

重构方法(Reconstitution)

See Certificate of Analysis (CoA) for detailed instruction.

存储(Storage)

Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

原理(Assay Principles)

Application Method: a) Reconstitute the Beads following the COA. Wash and re-suspend the beads to a certain concentration by adding your dilution buffer. b) Add the prepared beads to your samples. c) Beads can be separated from your samples afterwards using a magnetic plate.

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

制剂(Formulation)

Please contact us for detailed information.

Contact us for customized product form or formulation.

 

典型数据-Typical Data

Spike RBD TYPICAL DATA

Immobilized 73.1 μg Anti-RBD antibody/1mg beads can bind SARS-CoV-2 Spike RBD (Cat. No. SPD-C5259) with an EC50 of 0.4705 μg/mL.

Protocol

 

前沿进展

 
 
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