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Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)

For research use only.

IDComponentsSize
RAS009-C01Pre-coated SARS-CoV-2 Spike RBD Microplate1 plate
RAS009-C02SARS-CoV-2 Antibody Positive Control200 μL
RAS009-C03SARS-CoV-2 Antibody Negative Control200 μL
RAS009-C04HRP-SARS-CoV-2 Spike RBD 10 μg
RAS009-C0510xWashing Buffer 50 mL
RAS009-C06Dilution Buffer50 mL
RAS009-C07Substrate Solution12 mL
RAS009-C08Stop Solution7 mL

背景(Background)

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.

应用说明(Application)

This kit is developed for serologic test for Total Anti-SARS-CoV-2 antibody in serum/plasma in vitro.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储 & 运输(Storage & Shipping)

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Shipping Statement

原理(Assay Principles)

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of total anti-SARS-CoV-2 antibody in serum by SARS-CoV-2 Spike protein RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Spike RBD and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted HRP-SARS-CoV-2 Spike RBD to the plate. The HRP-SARS-CoV-2 Spike RBD is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

典型数据-Typical Data Please refer to DS document for The assay protocol.

Spike RBD TYPICAL DATA

Detection of Monoclonal Anti-SARS-CoV-2 Antibody (Spike RBD) titer by sandwich-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 0.5 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Antibody (Spike RBD). Detection was performed using HRP-SARS-CoV-2 Spike RBD with sensitivity of 195 ng/mL (QC tested).

 

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