膜杰作
Star Staining
分子别名（Synonym）
IL-17 RC,IL-17RC,IL17Rhom,IL-17 receptor C,IL-17RL,ZcytoR14
表达区间及表达系统（Source）
Human IL-17 RC, Fc Tag (ILC-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - His 465 (Accession # NP_703190.2).
Predicted N-terminus: Leu 21
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 75.9 kDa. The protein migrates as 90-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Human IL-17 RC, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) is more than 90% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL17A, Tag Free (Cat. No. ILA-H5219) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 5-160 ng/mL (QC tested).
Protocol
Immobilized Human IL-17F, His Tag (Cat. No. ILF-H5244) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 0.02-0.25 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human IL-17A Protein, Tag Free immobilized on CM5 Chip can Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 72.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Biotinylated Human IL-17A Protein, His,Avitag™ (Cat. No. ILA-H82Q1) captured on Biotin CAP - Series S sensor Chip can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 40.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human IL17A, His,Avitag (Cat. No. ILA-H82Q1) on SA Biosensor, can bind uman IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 13.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 92.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-17F, His Tag (Cat. No. ILF-H5244) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 100 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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背景（Background）
Interleukin-17 receptor C (IL-17 RC), also known as IL17Rhom, IL-17RL, is the receptor for IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RA. IL-17A activity is inhibited by IL-17RA, IL-17F is inhibited by IL-17RC, and a combination of soluble IL-17RA/IL-17RC receptors is required for inhibition of the IL-17F/IL-17A activity. Furthermore, activation of IL17RC can lead to the induction of expression of inflammatory chemokines and cytokines such as CXCL1.
