膜杰作
Star Staining
分子别名（Synonym）
CD38,T10,cADPr hydrolase 1
表达区间及表达系统（Source）
FITC-Labeled Human CD38, Fc Tag (Cat. No. CD8-HF255) is expressed from human HEK293 cells. It contains AA Val 43 - Ile 300 (Accession # P28907-1). It is the FITC labeled form of Human CD38 Protein, Fc Tag (Cat. No. CD8-H5255).
Predicted N-terminus: Val 43
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 56.3 kDa. The protein migrates as 65-75 under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
蛋白标记度（Protein Ratio）
The FITC to protein molar ratio is 3-5.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
FITC-Labeled Human CD38, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Anti-CD38 Antibody (Daratumumab), Human IgG1 at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human CD38, Fc Tag (Cat. No. CD8-HF255) with a linear range of 0.078-0.625 μg/mL (QC tested).
Protocol
Immobilized Monoclonal Anti-CD38 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human CD38, Fc Tag (Cat. No. CD8-HF255) with a linear range of 0.039-1.25 ug/mL (Routinely tested).
Protocol
背景（Background）
CD antigen CD38 is also known as ADP-ribosyl cyclase 1, which belongs to the ADP-ribosyl cyclase family. CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. These reaction products are essential for the regulation of intracellular Ca2+. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism. CD38 has been used as a prognostic marker in leukemia.
