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 >  Protein>4-1BB >41B-H82E6

Biotinylated Human 4-1BB / TNFRSF9 Protein, His,Avitag™(MALS verified)

分子别名(Synonym)

TNFRSF9,4-1BB,CD137,CDw137,ILA

表达区间及表达系统(Source)

Biotinylated Human 4-1BB, His,Avitag (41B-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # Q07011-1).

Predicted N-terminus: Leu 24

Request for sequence

蛋白结构(Molecular Characterization)

4-1BB Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 20.9 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

4-1BB SDS-PAGE

Biotinylated Human 4-1BB, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

4-1BB SEC-MALS

The purity of Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

4-1BB ELISA

Immobilized UreluMab, Human IgG4 at 1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) with a linear range of 1-15 ng/mL (QC tested).

Protocol

4-1BB ELISA

Immobilized Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate can bind Human 4-1BB Ligand, Fc Tag with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

4-1BB ELISA

Immobilized Utomilumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, His,Avitag (Cat. No. 41B-H82E6) with a linear range of 0.5-8 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

4-1BB SPR

Biotinylated Human 4-1BB Protein, His,Avitag (Cat. No. 41B-H82E6) captured on Biotin CAP-Series S sensor Chip can bind anti 4-1BB mAb with an affinity constant of 0.158 nM as determined in a SPR assay (Biacore T200) (Routinely tested) .

Protocol

 

背景(Background)

4-1BB is also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF2.

文献引用(Citations)

 

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4-1BB靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 9
中文全称:肿瘤坏死因子受体超家族成员9
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:71详情
最高研发阶段:临床三期
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