膜杰作
Star Staining
抗体来源（Source）
Biotinylated Mouse IgG1 Isotype Control (mAb) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | kappa
偶联（Conjugate）
Biotin
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Mouse
特异性（Specificity）
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
应用（Application）
| Application | Recommended Usage |
| ELISA | 1-150 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Mouse IgG1 Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) is more than 95% and the molecular weight of this protein is around 135-170 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) with a linear range of 1-39 ng/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Anti-CD19 CAR-293 cells were stained with a series of concentrations of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
5e5 of PBMCs were stained with a series of concentrations of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
背景（Background）
A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
