膜杰作
Star Staining
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
Binding assay by FACS and cell based ELISA.
应用说明（Application）
Human PD-L1, GFP Tag-HEK293 cells stably expressing exogenous human PD-L1 (Programmed cell death 1 ligand 1, CD274, B7-H1, PDCD1L1, PDCD1LG1, AA sequence : Leu 19 - Leu 238, Protein Acc.# : Q9NZQ7-1). At the same time, GFP protein as a marker was expressed in HEK239/hPD-L1 cells for screening positive cells by FACS.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
G418 (0.5mg/mL)
培养基（Culture Medium）
DMEM medium +10% FBS
冻存液（Freeze Medium）
20% FBS+10% DMSO+70% DMEM medium
装量（Quantity）
1 vial contains ~ 5x10^6 cells in 1 ml of 10% DMSO in (DMEM+22%FBS).
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1 μl stock solution per million cells (Routinely tested).
Protocol
FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti- human PD-L1 antibody. The IC50 is 0.29 μg/ml (Routinely tested).
Protocol
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Limited Use&License Disclosure
- This cell line is provided for research use only. It is neither intended for any animal or human therapeutic use nor for any direct human in vivo use. You are restricted to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
