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Biotinylated Human CA125 / MUC16 (13810-14451) Protein, Fc,Avitag™

分子别名(Synonym)

CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16

表达区间及表达系统(Source)

Biotinylated Human CA125 (13810-14451), Fc,Avitag (CA5-H82F8) is expressed from human 293 cells (HEK293). It contains AA Pro 13810 - Pro 14451 (Accession # Q8WXI7-1).

Predicted N-terminus: Pro 13810

Request for sequence

蛋白结构(Molecular Characterization)

CA125 Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 100.0 kDa. The protein migrates as 120-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CA125 SDS-PAGE

Biotinylated Human CA125 (13810-14451), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

CA125 ELISA

Immobilized Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H526x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CA125 (13810-14451), Fc,Avitag (Cat. No. CA5-H82F8) with a linear range of 0.6-78 ng/mL (QC tested).

Protocol

 

背景(Background)

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

 

前沿进展

 
 
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CA125靶点信息
英文全称:Mucin-16
中文全称:粘蛋白-16
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:6详情
最高研发阶段:临床三期
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