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 >  Protein>GDF-2 >GD2-H5211

Human GDF-2 Protein, Tag Free

分子别名(Synonym)

GDF-2,GDF2,BMP-9,BMP9

表达区间及表达系统(Source)

Human GDF-2, Tag Free (GD2-H5211) is expressed from human 293 cells (HEK293). It contains AA Ser 320 - Arg 429 (Accession # Q9UK05-1).

Predicted N-terminus: Ser 320

Request for sequence

蛋白结构(Molecular Characterization)

GDF-2 Structure

This protein carries no "tag".

The protein has a calculated MW of 12.1 kDa. The protein migrates as 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in ACN, TFA in Water with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

GDF-2 SDS-PAGE

Human GDF-2, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

GDF-2 ELISA

Immobilized Human GDF-2, Tag Free (Cat. No. GD2-H5211) at 1 μg/mL (100 μL/well) can bind Human ALK-1, His Tag with a linear range of 20-156 ng/mL (QC tested).

Protocol

 

背景(Background)

Human Growth and differentiation factor 2 (GDF-2), also known as Bone morphogenetic protein 9 (BMP-9), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. GDF-2 Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG. ALK1 is a signalling receptor for bone morphogenetic protein-9 (BMP-9) in endothelial cells (ECs). BMP-9 bound with high affinity to ALK1 and endoglin, and weakly to the type-I receptor ALK2 and to the BMP type-II receptor (BMPR-II) and activin type-II receptor (ActR-II) in transfected COS cells. Binding of BMP-9 to ALK2 was greatly facilitated when BMPR-II or ActR-II were co-expressed.

 

前沿进展

 
 
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GDF-2靶点信息
英文全称:Growth/differentiation factor 2
中文全称:骨形态发生蛋白-9
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
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