膜杰作
Star Staining
产品描述（Product Details）
This product is derived from Streptococcus pyogenes and expressed in E. coli, it is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
应用说明（Application）
IdeS cleaves IgG to produce F(ab')2 and Fc fragments at specific site.
单位定义（Unit Definition）
One unit digests ≥ 95% of 1 μg human IgG when incubated in 10 mM sodium phosphate, 137 mM NaCl, 2.7 mM KCl, pH7.4 at 37°C for 30min.
纯度（Purity）
>95% as determined by SDS-PAGE.
酶活（Enzyme Activity）
>70 U/μL
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, PH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）
Bioactivity of IdeS is confirmed by the cleavage of Human IgG as analyzed by SDS-PAGE (QC tested).
50/100unit Ides digests ≥ 95% of 100 µg human IgG when incubated in PBS pH 7.4 at 37℃ for 30min (Routinely tested).
50/100unit Ides digests ≥ 95% of 100 µg human IgG when incubated in PBS pH 7.4 at 37℃ for 30min (Routinely tested).
