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 >  Protein>SLAMF7 >SL7-HP2H3

PE-Labeled Human SLAMF7 / CRACC / CD319 Protein, His Tag (Site-specific conjugation)

分子别名(Synonym)

SLAMF7,CD319,CS1,CRACC,19A,FOAP-12

表达区间及表达系统(Source)

PE-Labeled Human SLAMF7, His Tag (SL7-HP2H3) is produced via site-specific conjugation of PE to Human SLAMF7, His Tag under optimal conditions with a proprietary technology. Human SLAMF7, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Met 226 (Accession # Q9NQ25-1).

Predicted N-terminus: Ser 23

Request for sequence

蛋白结构(Molecular Characterization)

SLAMF7 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 25.6 kDa.

偶联(Conjugate)

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

应用说明(Application)

Evaluation of anti-SLAMF7 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

活性(Bioactivity)-FACS

SLAMF7 FACS

1e6 of the anti-SLAMF7 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human SLAMF7, His Tag (Cat. No. SL7-HP2H3) and negative control protein respectively, PE signals was used to evaluate the binding activity (QC tested).

Protocol

背景(Background)

SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), Membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Isoform 1 of SLAMF7 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. Isoform 3 of SLAMF7 does not mediate any NK cell activation.

 

 

前沿进展

 
 
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SLAMF7靶点信息
英文全称:SLAM family member 7
中文全称:SLAM家族成员-7
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:5详情
最高研发阶段:批准上市
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