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 >  Protein>IgG Fc >IGG-R5203

Rabbit IgG Fc Protein, Tag Free (MALS verified)

分子别名(Synonym)

IgG Fc

表达区间及表达系统(Source)

Rabbit IgG Fc, Tag Free (IGG-R5203) is expressed from human 293 cells (HEK293). It contains AA Cys 105 - Lys 323 (Accession # P01870-1).

Predicted N-terminus: Cys 105

Request for sequence

蛋白结构(Molecular Characterization)

IgG Fc Structure

This protein carries no "tag". The protein has a calculated MW of 24.8 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IgG Fc SDS-PAGE

Rabbit IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IgG Fc SEC-MALS

The purity of Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) is more than 90% and the molecular weight of this protein is around 52-66 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IgG Fc ELISA

Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.3-5 ng/mL (QC tested).

Protocol

IgG Fc ELISA

Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

Protocol

 

背景(Background)

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport).

 

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