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 >  Protein>Axl >AXL-H5253

Human Axl Protein, Fc Tag (MALS verified)

分子别名(Synonym)

AXL,UFO

表达区间及表达系统(Source)

Human Axl, Fc Tag (AXL-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Trp 451 (Accession # P30530-1).

Predicted N-terminus: Ala 26

Request for sequence

蛋白结构(Molecular Characterization)

Axl Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 72.6 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, 25 mM Arginine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Axl SDS-PAGE

Human Axl, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Axl SEC-MALS

The purity of Human Axl, Fc Tag (Cat. No. AXL-H5253) is more than 90% and the molecular weight of this protein is around 165-200 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Axl ELISA

Immobilized Human GAS6, His Tag (Cat. No. GA6-H5249) at 5 μg/mL (100 μL/well) can bind Human Axl, Fc Tag (Cat. No. AXL-H5253) with a linear range of 0.1-10 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

Axl SPR

Human Axl, Fc Tag (Cat. No. AXL-H5253) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human GAS6, His Tag (Cat. No. GA6-H5249) with an affinity constant of 1.23 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

AXL Receptor Tyrosine Kinase is also known as Tyrosine-protein kinase receptor UFO, which belongs to the protein kinase superfamily, Tyr protein kinase family and AXL/UFO subfamily. AXL contains two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. AXL is highly expressed in metastatic colon tumors. AXL is activated by GAS6-binding and subsequent autophosphorylation. AXL is involved in signal transduction from the extracellular matrix into the cytoplasm by binding growth factors, and thus implicated in the stimulation of cell proliferation.

 

前沿进展

 
 
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Axl靶点信息
英文全称:AXL receptor tyrosine kinase
中文全称:AXL受体酪氨酸激酶
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:28详情
最高研发阶段:批准上市
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