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Mouse Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, His Tag&Tag Free (MALS verified)

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分子别名(Synonym)

Integrin alpha 2 beta 1,ITGA2 & ITGB1

表达区间及表达系统(Source)

Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-M52W3) is expressed from human 293 cells (HEK293). It contains AA Tyr 27 - Thr 1129 (ITGA2) & Gln 21 - Asp 728 (ITGB1) (Accession # Q62469-1(ITGA2) & P09055-1(ITGB1)).

Predicted N-terminus: Tyr 27 (ITGA2) & Gln 21 (ITGB1)

Request for sequence

蛋白结构(Molecular Characterization)

Online(Tyr 27 - Thr 1129 (ITGA2) & Gln 21 - Asp 728 (ITGB1)) Q62469-1 (ITGA2) & P09055-1 (ITGB1)

Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.5 kDa (ITGB1). Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 140-150 kDa (ITGA2) and 100-130 kDa (ITGB1) respectively due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-M52W3) SDS-PAGE gel

Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-M52W3) MALS images

The purity of Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-M52W3) is more than 90% and the molecular weight of this protein is around 210-240 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Collagen IV at 2 μg/mL (100 μL/well) can bind Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-M52W3) with a linear range of 0.02-0.625 μg/mL (QC tested).

Protocol

背景(Background)

Integrin alpha 2 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit. It is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A and Human echoviruses 1 and 8. DGEA inhibited rotavirus binding to alpha2beta1 and infectivity. In a novel process, integrin-using viruses bind the alpha2 I domain of alpha2beta1 via DGE in VP4 and interact with alphaXbeta2 (via GPR) and alphaVbeta3 by using VP7 to facilitate cell entry and infection.

 

 

前沿进展

 
 
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Integrin alpha 2 beta 1靶点信息
英文全称:Integrin alpha2/beta1
中文全称:整合素α-2/β-1复合体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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