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 >  Protein>Gastrin-17 >GA7-H82F4

Biotinylated Human Gastrin-17 Protein, Fc,Avitag™ (MALS verified)

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分子别名(Synonym)

gastrin-17,gastrin 17,gastrin

表达区间及表达系统(Source)

Biotinylated Human Gastrin-17, Fc,Avitag (GA7-H82F4) is expressed from human 293 cells (HEK293).

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 30.3 kDa. The protein migrates as 34-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

无菌(Sterility)

Negative

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Gastrin-17 SDS-PAGE

Biotinylated Human Gastrin-17, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Gastrin-17 SEC-MALS

The purity of Biotinylated Human Gastrin-17, Fc,Avitag (Cat. No. GA7-H82F4) is more than 90% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Gastrin-17 ELISA

Immobilized Anti-Gastrin-17 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human Gastrin-17, Fc,Avitag (Cat. No. GA7-H82F4) with a linear range of 0.1-2.5 ng/mL (QC tested).

Protocol

 

背景(Background)

Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the pyloric antrum of the stomach, duodenum, and the pancreas. Gastrin binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+/H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release into the stomach cavity). Its release is stimulated by peptides in the lumen of the stomach.

 

前沿进展

 
 
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Gastrin-17靶点信息
英文全称:Gastrin 17
中文全称:促胃泌素 17
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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