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 >  Protein>LOXL2 >LO2-H52H3

Human LOXL2 Protein, His Tag (active enzyme)

分子别名(Synonym)

Lysyl oxidase homolog 2,LOXL2

表达区间及表达系统(Source)

Human LOXL2, His Tag (LO2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Gln 774 (Accession # Q9Y4K0-1).

Predicted N-terminus: Gln 26

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gln 26 - Gln 774) Q9Y4K0-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 86.0 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 20 mM MES, 50 mM NaCl, pH6.5 with trehalose as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

Human LOXL2, His Tag (Cat. No. LO2-H52H3) SDS-PAGE gel

Human LOXL2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)

Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is >5 pmol/min/μg (QC tested).

Protocol

 

背景(Background)

Lysyl oxidase homolog 2 is also known as LOXL2, Lysyl oxidase-like protein 2, which is expressed in many tissues, highest expression in reproductive tissues, placenta, uterus and prostate, Up-regulated in a number of cancers cells and tissues. LOXL2 mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. LOXL2 acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation.

 

 

前沿进展

 
 
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LOXL2靶点信息
英文全称:Lysyl oxidase homolog 2
中文全称:赖氨酰氧化酶同源蛋白-2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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