膜杰作
Star Staining
| Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Physical appearance | Powder mixture | Powder mixture |
| Amount of Coupled Protein | ≈261 pmol (11 μg) EGFRvIII/mg beads | ≈261 pmol (11 μg) EGFRvIII/mg beads |
| Binding Capacity | >200 pmol (30 μg) antibody/mg beads | >200 pmol (30 μg) antibody/mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
背景（Background）
The Human EGFRvIII-coupled Magnetic Beads is produced by coupling biotinylated EGFRvIII protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-EGFRvIII antibody from your sample with high specificity.
表达区间及表达系统（Source）
Human EGFRvIII Protein is expressed from human 293 cells (HEK293). It contains AA Leu 25- Ser 378(Accession # NP_001333870.1).
应用说明（Application）
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the beads at -20°C. The shelf life is 6 months at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data
Immobilized 44 μg EGFRvIII protein/1 mg beads can bind Anti-EGFR Antibody (Cetuximab) with an EC50 of 0.7172 μg/mL.
Protocol
Immobilized 44 μg EGFRvIII protein/1mg beads can bind Anti-EGFRvIII Antibody with an EC50 of 0.8856 μg/mL (Routinely tested).
Protocol
