膜杰作
Star Staining
& Cat.No. | Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Physical appearance | Powder mixture | Powder mixture |
| Amount of Coupled Protein | ≈1194 pmol (33.8 μg) SARS-CoV-2 RBD protein/mg beads | ≈1194 pmol (33.8 μg) SARS-CoV-2 RBD protein/mg beads |
| Binding Capacity | >300 pmol (33 μg) Human ACE2 ligand/mg beads | >300 pmol (33 μg) Human ACE2 ligand/mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
背景（Background）
The SARS-CoV-2 Spike RBD (B.1.1.7) Coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike RBD to streptavidin-conjugated magnetic beads. The spike RBD coupled to the beads contains the critical N501Y mutation identified in multiple circulating SARS-CoV-2 variants, e.g. the U.K. variant (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01), the South African variant (known as B.1.351 or 20C/501Y.V2) and the Brazilian variant (known as P.1). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
表达区间及表达系统（Source）
SARS-CoV-2 Spike RBD (B.1.1.7) Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (N501Y)).
应用说明（Application）
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data
Immobilized 33 μg RBD protein (Cat. No. SPD-C82E6) /1mg beads can bind ACE2 (Cat. No. AC2-H5257) with an EC50 of 1.337 μg/mL.
Protocol
Immobilized 56 μg SARS-CoV-2 Spike RBD (B.1.1.7)/1 mg beads can bind Anti-S1 Ab (Cat. No. S1N-M122) with an EC50 of 0.1069 μg/mL.
Protocol
