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 >  Protein>NCAM-1 >NC1-HF256

FITC-Labeled Human NCAM-1 / CD56 Protein, Fc Tag

分子别名(Synonym)

CD56,MSK39,NCAM1,N-CAM-1

表达区间及表达系统(Source)

FITC-Labeled Human NCAM-1, Fc Tag (NC1-HF256) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Gly 718 (Accession # P13591-2).

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蛋白结构(Molecular Characterization)

Online(Leu 20 - Gly 718) P13591-2

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 104.0 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 1.5-3.5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FITC-Labeled Human NCAM-1, Fc Tag (Cat. No. NC1-HF256) SDS-PAGE gel

FITC-Labeled Human NCAM-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-NCAM-1 Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human NCAM-1, Fc Tag (Cat. No. NC1-HF256) with a linear range of 0.08-0.625 μg/mL (QC tested).

Protocol

背景(Background)

NCAM1 belongs to the immunoglobulin superfamily of adhesion molecules. A wide range of alternatively spliced NCAM1 messenger RNAs (mRNAs) has been described to date, but only the 120-, 140-, and 180- kDa isoforms are commonly expressed. NCAM1 plays an important role in the regulation of neurogenesis, neurite outgrowth, proliferation, and cell migration, however, its function in hematopoiesis, including NK cells, is poorly understood. NCAM1 signaling is mediated either by homophilic or heterophilic interactions with fibroblast growth factor receptor (FGFR), L1-CAM, N-cadherin and other components of the extracellular matrix. Upon activation, NCAM1 triggers a variety of signaling cascades including FYN–focal adhesion kinase (FAK), MAPK, and phosphatidylinositol 3-kinase (PI3K) pathways.

 

 

前沿进展

 
 
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NCAM-1靶点信息
英文全称:Neural cell adhesion molecule 1
中文全称:神经细胞粘附分子-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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