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 >  Protein>Nectin-4 >NE4-R5255

Rat Nectin-4 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

NECTIN4,LNIR, PRR4, PVRL4

表达区间及表达系统(Source)

Rat Nectin-4, Fc Tag (NE4-R5255) is expressed from human 293 cells (HEK293). It contains AA Tyr 28 - Val 348 (Accession # D3ZET1-1).

Predicted N-terminus: Tyr 28

Request for sequence

蛋白结构(Molecular Characterization)

Nectin-4 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 61.1 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Nectin-4 SDS-PAGE

Rat Nectin-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Nectin-4 SEC-MALS

The purity of Rat Nectin-4, Fc Tag (Cat. No. NE4-R5255) is more than 90% and the molecular weight of this protein is around 125-140 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Nectin-4 ELISA

Immobilized Rat Nectin-4, Fc Tag (Cat. No. NE4-R5255) at 1 μg/mL (100 μL/well) can bind Anti-Nectin-4 Antibody, Mouse IgG1 with a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

 

背景(Background)

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.

 

前沿进展

 
 
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Nectin-4靶点信息
英文全称:Nectin-4
中文全称:细胞黏附分子nectin-4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:10详情
最高研发阶段:批准上市
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