膜杰作
Star Staining
分子别名（Synonym）
FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
表达区间及表达系统（Source）
HRP-Human CD3 epsilon, His Tag (CDE-HR2H4) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).
Predicted N-terminus: Asp 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 12.6 kDa.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized Mouse Anti Human CD3(SP-34) at 1 μg/mL (100 μL/well) can bind HRP-Human CD3 epsilon, His Tag (Cat. No. CDE-HR2H4) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Human BCMA, His Tag (Cat. No. BCA-H522y) at 1 μg/mL (100 μL/well) can bind Bispecific BCMA×CD3 T cell-engaging Antibody with a linear range of 5-39 ng/mL when detected by HRP-Human CD3 epsilon, His Tag (Cat. No. CDE-HR2H4) (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Bispecific T-cell Engager captured on Protein A Chip can bind HRP-Human CD3 epsilon, His Tag (Cat. No. CDE-HR2H4) with an affinity constant of 4.45 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
