膜杰作
Star Staining
分子别名（Synonym）
IL-17A,Interleukin-17A,CTLA-8,IL-17
表达区间及表达系统（Source）
Canine IL-17A, His Tag (ILA-C52H7) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Ala 155 (Accession # C6L8D7-1).
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 18 kDa and 22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Canine IL-17A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-Human IL17A Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Canine IL-17A, His Tag (Cat. No. ILA-C52H7) with a linear range of 5-39 ng/mL (QC tested).
Protocol
Immobilized Canine IL-17A, His Tag (Cat. No. ILA-C52H7) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 4-63 ng/mL (Routinely tested).
Protocol
Immobilized Canine IL-17A, His Tag (Cat. No. ILA-C52H7) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (MALS verified) with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
背景（Background）
Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
